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NEW TempliPhi Sequence Resolver Kit buy illustra PlasmidPrep MiniTempliPhi Sequence Resolver Kit NEW NEW

Simplify Difficult Template Sequencing

INTRODUCING THE EASIEST SOLUTION FOR DNA TEMPLATE PREPARATION

TempliPhi will save you time by eliminating the need for culture growth.  It's simple to use and gives you high quality results.

From picogram starting amounts, TempliPhi™ DNA Amplification Kits generates microgram quantities of template DNA utilizing bacteriophage Phi29 DNA polymerase and rolling circle amplification. This unique approach eliminates the need for culture growth, giving you high quality DNA templates in just four to six hours—with less than 20 minutes of hands-on-time. Uniform quantities of DNA are amplified in all samples, so there's no need to quantify each sample before sequencing—saving you even more time and effort.

Amplified DNA can be used directly for sequencing using any chemistry and sequencing platform. Amplified DNA can also be processed by restriction enzymes and stored for future needs. What's more, it makes automation easy.

TempliPhi is easy to use, simple protocols suit all applications, and is available in 100, 500 and 10 000 reaction kits.

See an animation explaining rolling circle amplification.
Find out more about rolling circle amplification.
TempliPhi™ DNA Sequencing Template Amplification Kits


Find TempliPhi citations on Google Scholar: Click here to search.

Read the Methods:
Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification
Genome Res. - Vol. 11, Issue 6, 1095-1099, June 2001