Cell Separation Media handbook contains methodology and applications

Since its introduction in 1977, the silica colloid Percoll™ has become the density gradient medium of choice for thousands of researchers worldwide. Its nearly ideal physical characteristics facilitate its use in separating cells, organelles, viruses, and other subcellular particles. Percoll is especially useful as a first step to enrich for cell populations before attempting finer resolution or extraction of nucleic acids. A considerable savings of time and resources may be realized using Percoll as a first step before employing these methods. This re-issued manual provides the basic methodology for making and using gradients of Percoll and includes information on Percoll PLUS, a new silica based colloidal medium optimized for cell separation in clinical research applications. In addition, the Application Tables in the latter part of this manual provide numerous references for using Percoll to isolate various cells, microorganisms, organelles and subcellular particles. All experiments described in the literature using Percoll can also be performed with Percoll PLUS.

Download Cell Separation Media handbook