Comparison of ECL Plex, ECL Advance™, ECL Plus™ and ECL™These results were produced in a model system using recommended protocols with optimized antibody concentrations.
Target: human apotransferrin two-fold dilutions starting at 5 ng.
Detection: ECL Plex™, Typhoon™ 9410; all others, Hyperfilm™ ECL.
Primary antibody: rabbit polyclonal anti-human transferrin.
Secondary antibody: ECL Plex, ECL Plex goat-a-rabbit-Cy™5; all others, donkey-a-rabbit IgG (HRP-linked).
ECL Plus chemifluorescent detection: linearity, R2 = 0.994; dynamic range = 2.1; sensitivity = 39 pg.
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