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GENE EXPRESSION
High-quality microarray data using CodeLink Bioarray Platform
February 2003
P. Stafford, T. Sendera, T. Kaysser-Kranich, and C. Palaniappan
Amersham Biosciences, Chandler, AZ, USA
Abstract
CodeLink™ Bioarray Platform offers a high-precision microarray solution in a high-density format. Presynthesized and functionally validated oligonucleotide probes are piezo-electrically deposited onto a proprietary 3-D aqueous gel matrix. The matrix enables CodeLink Bioarrays to mimic solution-phase hybridization kinetics and deliver superior results. The platform achieves a sensitivity down to approximately one transcript per cell, and a minimum detectable fold change as low as 1.3-fold with 95% confidence. Probe specificity is estimated at 90% probe homology to target sequence. The CodeLink Platform includes expression bioarrays for human, rat, and mouse; a rat ADME bioarray, and a P450 bioarray for SNP analysis.
Fig 1. The CodeLink Bioarray Platform. Clockwise from upper left, a four-chamber SNP bioarray, a single-chamber expression bioarray, the 12-cell stackable hybridization tray, the 37 °C hybridization shaker, and the wash station.
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