Life Science News

HLA RSCA Typer 1.0 Software

• High resolution HLA typing

• Requires only one PCR that can be stored for re-analysis

• Simple and rapid method requires only 30 minutes for PCR^v set-up, 30 minutes for gel casting, and a few minutes for HLA assignment

• New allele discoveries do not require kit changes—they are handled by HLA RSCA Typer Software updates

1. An amplification of an HLA class of interest;

2. Part of the amplified DNA is then mixed with two or three reference strands and denatured;

3. After re-annealing and heteroduplex formation, fragment analysis is performed using a non-denaturing gel on ALFexpress II.

4. HLA assignment is made automatically using the new RSCA HLA Typer software together with ALFwin™ Fragment Analyser.