| Affinity chromatography (AC) is a technique enabling purification of a biomolecule with respect to biological function or individual chemical structure. The substance to be purified is specifically and reversibly adsorbed to a ligand (binding substance), immobilized by a covalent bond to a chromatographic bed material (matrix). Samples are applied under favourable conditions for their specific binding to the ligand. Substances of interest are consequently bound to the ligand while unbound substances are washed away. Recovery of molecules of interest can be achieved by changing experimental conditions to favour desorption. Affinity chromatography media are commonly used for applications such as purification of fusion proteins, mono- and polyclonal antibodies, and glycoproteins.
GE Healthcare offers a comprehensive range of affinity chromatography columns and media covering most current applications. The animation, selection guide, handbook, FAQ's, and education centre on these pages are designed to assist you when making a selection of affinity chromatography media for a specific application. If you cannot find the products you require on these pages, please contact us for a personal consultation with the experts in chromatography columns and media.
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