| Working principle |
- Separates molecules according to size.
|
| Used for |
- Sample conditioning (desalting, buffer exchange).
- Removal of low Mr or high Mr contaminants.
- Separation of complex samples.
|
| Suitable stage in a purification protocol |
- Capture:
- Intermediate:
- Polishing:
  
- Sample Conditioning:
  
(Number of stars indicate suitability.) |
| Separation characteristics |
|
| Moderate |
|
| - General separation: Limited
- Sample conditioning: High
. |
|
| Moderate to low
. |
|
| Very High |
|
| High |
|
| Main optimizing parameter |
|
| Special features |
- Almost any buffer can be used.
- The only separation technique separating according to size.
- Applicable to labile proteins.
|
| Further reading |
- Applications
- Media selection guide
- Purification protocols
- Purification strategies
- Gel Filtration handbook
|
