1.
Equilibration.
Buffer A is pumped through the column until conditions match those of buffer A. | 2.
Sample application and wash.
Sample is added and adsorbed and non-adsorbed components are washed out. | 3.
Gradient elution.
Gradient is started and adsorbed sample components are eluted in order of increasing hydrophobicity.
Elution order:
   | 4.
Regeneration.
Hydrophobic contaminants still remaining are washed out.
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