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GE Healthcare Life Sciences Part of GE Healthcare
Education Centre
About the purification of biomolecules
Purpose of purification
Developing purification protocols
How to combine purification steps
Purification development - summary
LC techniques
Affinity Chromatography
Desalting & Gel Filtration
Hydrophobic interaction chromatography
Ion exchange chromatography
Animation
Basic Principles
Ion Exchange in practice
Technique Profile
What is Ion Exchange?
Reversed phase chromatography
Protein Purifier software
Tutorial
BioProcess™ Glossary

Ion exchange chromatography profile

Working principle
  • Separates molecules according to charge
Used for
  • Separation of complex samples.
  • Sample concentration.
  • Removal of charged contaminants.
  • Separation of complex samples.
Suitable stage in a purification protocol
  • Capture:
  • Intermediate:
  • Polishing:
(Number of stars indicate suitability.)
Separation characteristics
  • Resolution
High
  • Load capacity
- General separation: High
- Group separation: Very High
- Sample concentration: Very High
.
  • Speed
- General separation: High
- Group separation: Very High
- Sample concentration: Very High
.
  • Gentleness
High
  • Mass yield.
High
Main optimizing parameter
    Choice of running pH.
Special features
  • Selectivity can be varied over a wide range.
  • Wide applicability to proteins and peptides.
Further reading
    - Applications
    - Media selection guide
    - Purification protocols
    - Purification strategies
    - (Handbooks etc.)