| Based on the hydrophobic interactions between solute molecules and immobilized, matrix-bound ligands, reversed phase chromatography (RPC) has found favour in a wide variety of preparative applications including micropurification of protein fragments for sequencing and process-scale purification of recombinant protein products. Reversed phase chromatography relies on a partitioning mechanism between the solute molecules of the sample mobile phase and the stationary phase (matrix) in order to achieve separation. Retention of molecules of interest can be controlled by manipulating the properties of the mobile phase, and separation of molecules with only small differences in hydrophobicity can be performed. Reversed phase chromatography also offers the benefit of exceptional flexibility in separation conditions so that either molecules of interest can be bound while contaminants pass through, or contaminants can be bound while the molecule of interest passes through.
The animation, selection guide, handbook, FAQ's, and education centre on these pages are designed to assist you when making a selection of reversed phase chromatography media for a specific application. If you cannot find the products you require on these pages, please contact us for a personal consultation with the experts in chromatography columns and media. |
