GE Healthcare
 
GE Healthcare Life Sciences Part of GE Healthcare
labsep
Education Centre
About the purification of biomolecules
Purpose of purification
Developing purification protocols
How to combine purification steps
Purification development - summary
LC techniques
Affinity Chromatography
Desalting & Gel Filtration
Hydrophobic interaction chromatography
Ion exchange chromatography
Reversed phase chromatography
Animation
Basic principles
The Separation Mechanism
Elution modes
The typical RPC experiment
The mobile phase
The stationary phase
Resolution in RPC
Optimisation of RPC experiments
RPC in practice
Technique Profile
What is RPC?
Protein Purifier software
BioProcess™ Glossary
Basic principles in reversed phase chromatography

Content
1.The Separation Mechanism
- Solubility properties
- Adsorption mechanisms
- Hydrophobic interaction
- Desorption curves
2.Elution modes
- Isocratic elution
- Gradient elution
3.The typical RPC experiment
4.The mobile phase
- The eluent
- Eluent pH
- Ion pairing agents
5.The stationary phase
6.Resolution in RPC
7.Optimisation of RPC experiments