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Addition of imidazole during binding improves purity of histidine-tagged proteins

The purity of histidine-tagged proteins purified by metal chelate affinity chromatography can often be improved by optimizing the imidazole concentration in the sample and binding buffer to achieve a ...

Automated Multistep Purification of (His)6 and GST-tagged Proteins

Automated multistep purifi cations of five affinity tagged proteins were performed using ÄKTAxpress™, a new chromatography system. The following purification results are presented: • One ...

His MultiTrap FF and His MultiTrap HP prepacked 96-well filter plates - for reproducible purification screening of histidine-tagged proteins

The new prepacked 96-well filter plates, His MultiTrap™ FF and His MultiTrap HP, offer convenient screening and rapid high-throughput purification screening of histidine-tagged proteins. His ...

HisTrap FF crude for faster purifcation of histidine-tagged proteins

Features of HisTrap™ FF crude include: No need for centrifugation or filtration of the samples. Direct sample application. No difference in final purity and recovery when using: – clarified or ...

Optimizing Protocols for Automated Multistep Purification of (histidine)6-tagged Protein using ÄKTAxpress

ÄKTAxpress™ is a chromatography system for automated multistep purification of large numbers of (histidine)6- and Glutathione S-transferase (GST)-tagged proteins. Pre-optimized methods are easily ...

Reproducible and robust peptide separations using Ettan MDLC, a multidimensional liquid chromatography system

This application note demonstrates the reproducibility and robustness of Ettan MDLC for high-throughput one-dimensional (1D) LC analysis and two-dimensional (2D) LC analysis, and highlights the ...