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ÄKTA 3D plus Kit used for automated multistep purification of antibodies

ÄKTA™ 3D plus Kit is designed for automated multistep purification of affinity-tagged proteins as well as antibodies. With automated purification, each purified sample can contain up to 50 mg of ...

Automated Multistep Purification of (His)6 and GST-tagged Proteins

Automated multistep purifi cations of five affinity tagged proteins were performed using ÄKTAxpress™, a new chromatography system. The following purification results are presented: • One ...

Automated multistep purification of histidine-tagged proteins from unclarified cell lysates

HisTrap™ FF crude is a ready to use column intended for purification of histidine-tagged proteins from unclarified cell lysates. No centrifugation or filtration of the samples is required before ...

Automated purification and quantitation of soluble histidine-tagged integrase of Tn21 from E. coli cells predominantly expressing the protein as inclusion bodies

The class I integron integrase of Tn21 is a basic DNAbinding protein with a theoretical pI value of 10.25 and a relative molecular weight of 38 000. This integrase belongs to the tyrosine family of ...

Automated purification of dual-tagged proteins

Recombinant proteins can be designed to contain N- or C-terminal affinity tags. If including two different tags in a construct, the target protein can be purified utilizing different affinity media, ...

High-throughput purification of antibodies

ÄKTAxpress™ MAb is a chromatography system for high throughput purification of antibodies. One and two step protocols including cleaning-in-place (CIP) procedures can easily be generated by a method ...

His MultiTrap FF and His MultiTrap HP prepacked 96-well filter plates - for reproducible purification screening of histidine-tagged proteins

The new prepacked 96-well filter plates, His MultiTrap™ FF and His MultiTrap HP, offer convenient screening and rapid high-throughput purification screening of histidine-tagged proteins. His ...

New prepacked 96-well filter plates, His MultiTrap and GST MultiTrap, for purification screening of Histidineand GST-tagged proteins

Efficient and rapid screening of recombinant proteins is an essential step for many applications in the areas of protein expression, function, and structure research. Therefore new ready-to-use ...

Optimization of the Elution Protocol for Recombinant Human Serum Albumin expressed in Pichia pastoris on Capto MMC using Design of Experiments

The multimodal resin, Capto™ MMC, is a novel cation exchange type of chromatography resin that maintains its protein binding capacity at high conductivity. Due to the multimodal nature of the ligand, ...