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Location: Protein Purification - Laboratory research > All News > Automated Protein Purification for Structural Studies
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Pär Eklund, Markus Galin, Pia Liljedahl, Christine Markeland Johansson,Thomas Pless, Jill A. Sigrell and Karin Torstenson International Genomic/Proteomic Technology, Summer Issue Structural studies require fairly large amounts (milligram scale) of pure and homogeneous target proteins. To ease the enormous task of performing these studies in a high-throughput mode, a reliable and easy-to-handle purification system is needed. This paper describes the automated purification of (His)6 and glutathione S-transferase (GST)-tagged recombinant proteins using the ÄKTA™ 3D kit with the ÄKTAexplorer™ 100 chromatography system (Amersham Biosciences, Uppsala, Sweden). With this combination, it is possible to automatically purify up to six proteins within a single day. PDF of article |