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Hydrophobic Interaction and Reversed Phase Chromatography: Principles and Methods

  • New handbook covers both hydrophobic interaction chromatography (HIC) and reversed phase chromatography (RPC)
  • Easy-to-follow, step-by-step procedures
  • Numerous examples featuring the latest HIC and RPC media

HIC and RPC are both are based upon interactions between hydrophobic patches on the surface of biomolecules and the hydrophobic surfaces of a chromatography medium. However, in practice, the methods are very different. This new, 168-page handbook describes both theoretical and practical aspects of the methods, the media available, and how to select them, together with application examples and detailed instructions for the most commonly performed procedures. Practical information, with many tips and hints drawn from over 40 years of experience in chromatography purification, guides beginners and experts toward obtaining the best possible results from the latest chromatography media.

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