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Purification of histidine-tagged recombinant proteins made more reliable
with new affinity chromatography medium
New pre-charged Ni Sepharose™ High Performance medium offers highest binding capacity on the market today
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| Ni Sepharose High Performance is available as 25 ml and 100 ml lab packs , but also as pre-packed HisTrap HP 1 ml and 5 ml columns.
Click here and see an animated presentation the benefits.
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. A new affinity chromatography medium offers optimized purification of histidine-tagged proteins. Ni Sepharose™ High Performance provides better protein binding capacity, negligible Ni leakage and compatibility with a wide range of reducing agents, detergents, denaturants and other additives.
The 34 µm bead matrix offers the highest binding capacity available on the market today to provide more concentrated samples, higher efficiency, time savings and increased yield. With negligible Ni leakage, the medium minimizes protein precipitation, and increases purity as well as yield.
Allan Simpson, Vice President Laboratory Separations at Amersham Biosciences comments, “Ni Sepharose High Performance is optimized for the purification of histidine-tagged proteins. Scientists in industrial and academic labs utilizing this new medium from Amersham Biosciences will have no difficulty translating its advantages into to greater purity, higher yields and increased activity, as well as greater operational flexibility.”
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 | HisTrap HP Kit is a rapid and convenient choice for one-step purifications of histidine-tagged proteins. |
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In addition to lab packs, Ni Sepharose High Performance is available as pre-packed HisTrap™ HP columns and HisTrap HP Kit for convenience and time-saving advantages. These HisTrap HP columns can be used with a syringe, a laboratory pump or chromatography systems such as ÄKTA™design from Amersham Biosciences.
Links to catalog
HisTrap HP
HisTrap HP Kit
Ni Sepharose High Performance
Link to Data file
Link to HiTrap column centre
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