 |
HisTrap FF crude Kit brings greater convenience to histidine-tagged protein purification
- Saves time in purifying histidine-tagged proteins and maintains the activity of your target protein
- Included HisTrap™ FF crude columns allow direct loading of unclarified cell lysates
- Contains three 1-ml HisTrap FF crude columns, buffer concentrates, connectors, syringe, and instructions
- Using a syringe possible—minimal backpressure even when loading unclarified cell lysates directly
| |
Buffer concentrates included in HisTrap FF crude Kit speed up the rate at which results are obtained, which increases simplicity and reproducibility. Preparing sample, binding, and elution buffers and then performing purification is done in a matter of minutes starting from an unclarified cell lysate. Purification can be performed simply using the syringe provided in the kit.
The ready-made buffer concentrates comprise:
- Phosphate binding buffer, pH 7.4 (2 × 50 ml, 8× concentrate)
- 2 M imidazole elution buffer concentrate, pH 7.4
The easy-to-follow instructions include:
- Sample preparation, purification, and optimization protocols
- Troubleshooting guide
For more information on HisTrap and HiTrap columns, click here.
Customer feedback on Ni Sepharose 6 Fast Flow.
|
| . |  |
.
|
