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Affinity - Coupling ligands
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Columns & Media
Affinity - Coupling ligands
Affinity - Group specific
Affinity-Isolation and Purification of Immunoglobulins
Affinity-Metal Chelate (IMAC)
Affinity - Tagged proteins
Custom Products
Desalting
Gel filtration
Hydrophobic Interaction
HiTrap columns
Ion Exchange
Reversed Phase 1
Reversed Phase 2
Prepacked columns
Chromatography Column Spare Parts
C columns
HR columns
SR columns
Tricorn
XK columns
Chromatography Columns & Media
Affinity - group specific
Affinity - antibody
Affinity - coupling
Affinity - immobilized lectin
Affinity - metal chelate
Affinity, nucleic acid, nucleotide and plasmid
Chromatofocusing
Custom Products
Desalting
Empty columns
Gel Filtration
Hydrophobic Interaction
Ion Exchange
Reversed Phase
Tagged protein purification
Chromatography Instruments
Accessories
Fraction Collectors
Mixers
Monitors
Pumps
Recorders
Software
Systems
Membrane separations
Hollow fiber cartridges
Kvick cassettes and holders
Systems
Media and pre-packed columns for coupling ligands
Product
Capacity/ substitution per ml medium
Spacer*
Groups to be coupled
Pre-packed columns
HiTrap
™
NHS-activated HP
10 mmole NHS groups
10-atom
-NH
2
Media
NHS-activated Sepharose
™
4 Fast Flow
16-23 mmole NHS groups
14-atom
-NH
2
CNBr-activated Sepharose 4 Fast Flow
13-26 mg
a-chymotrypsinogen
None
-NH
2
CNBr-activated Sepharose 4B
25-60 mg
a-chymotrypsinogen
None
-NH
2
Activated CH Sepharose 4B
>8 mmole glycyl-leucine
8-atom
-NH
2
ECH Sepharose 4B
12-16 mmole carboxyl groups
10-atom
-NH
2
Epoxy-activated Sepharose 6B
19-40 mmole epoxy groups
12-atom
-NH
2
-OH
-SH
EAH Sepharose 4B
7-11 mmole amino groups
11-atom
-COOH
Activated Thiol Sepharose 4B
1 mmole activated thiol groups
9-atom
-SH
Thiopropyl Sepharose 6B
18-31 mmole activated thiol groups
4-atom
-SH
*)
Spacer arms are used when coupling small molecules (M
r
<1000).
Spacer arms are generally not used for larger molecules (M
r
>5000) as they can contribute to non-specific binding.