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An efficient and rapid protein purification and on-column cleavage strategy using GSTrap Columns

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Automated tag removal using ÄKTAxpress (Life Science News 18, p.6-7)

ÄKTAxpress™ chromatography system, an integrated solution for high-throughput purification of His- and GST-tagged proteins, features methods for automated on-column removal of affinity tags. This ...

Easy purification of GST-tagged proteins (Discovery Matters 6, Aug 2007)

GE Healthcare offers a variety of affinity chromatography products for purification of GST-tagged proteins. The purification products described here are an integral part of the GST Genen Fusion ...

Fast and simple purification of GST fusion proteins using prepacked GSTrap affinity columns (from Life Science News, issue 4)

GSTrap™ affinity columns are prepacked, ready-to-use 1 ml and 5 ml HiTrap™ columns containing Glutathione Sepharose™ 4 Fast Flow. They are designed for one-step purification of glutathione ...

High-throughput purification of His- and GST-tagged proteins (from Life Science News Issue 17, August 2004)

ÄKTAxpress™ chromatography system is an integrated solution for high-throughput purification of His- and GST-tagged proteins. The system combines hardware, software and specially designed media to ...

Improved purification of GST-tagged proteins (from Life Science News Issue 16, February 2004)

Glutathione Sepharose™ High Performance is a new affinity medium for the purification of GST-tagged proteins. Designed for high-resolution purification, Glutathione Sepharose High Performance ...

Overcoming protein instability problems during fusion protein cleavage

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Screening for optimal GST-tagged protein buffer conditions using GST MultiTrap FF 96-well filter plates (from Discovery Matters Issue 4, June 2006)

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Separation of DnaK from pGEX-GST by ion exchange chromatography

DnaK (the 70 kDa protein produced by the gene dnaK) is a common contaminant of GST fusion proteins produced in E. coli, even after Glutathione Sepharose™ affinity chromatography. DnaK can be removed ...

Two-step, automated purification of GST-hippocalcin using GSTrap 4B and HiLoad 16/60 Superdex 200 pg columns on ÄKTAxpress chromatography system (from Discovery Matters Issue 4, June 2006)

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