GE Healthcare Life Sciences - Application notes

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A Novel Rapid Procedure for Purification of IgY from Egg Yolk

There are several advantages with the production of avian antibodies. The immunisation of chickens induces the production of specific antibodies in both serum and egg yolk in similar concentrations. ...

Continuous Perfusion: enabling maximum protein output

Most suspension-adapted cell culture processes are operated in either batch or fed-batch mode for a period of several days. Cell densities increase, metabolites are consumed and toxic waste products ...

MabSelect SuRe studies on ligand toxicity, leakage, removal of leached ligand, and sanitization

MabSelect SuRe™ is an affinity medium designed for purification of large volumes of monoclonal antibodies. The alkali-stabilized protein A-derived ligand allows extensive use of sodium hydroxide for ...

One-step purification of monoclonal IgM from cell culture supernatant

The development of a general purification protocol for immunoglobulin M (IgM) has met with problems, as the unique variation in the amino acid sequence of the variable regions give different ...

Optimization of loading conditions on Capto adhere using design of experiments - Process-scale antibody purification

Capto™ adhere is a strong ion exchanger with multimodal functionality designed for intermediate purification and polishing of monoclonal antibodies. Removal of remaining contaminants is achieved in ...

Process-scale purification of monoclonal antibodies – polishing using Capto Q

Anionic exchange media are an industry standard for largescale polishing of monoclonal antibodies (MAbs). Polishing is typically the last step in the purification process after Protein A and cationic ...

Purification of a Murine IgG1 Antibody Expressed in the Leaves of Arabidopsis Thaliana

Monoclonal antibodies are mostly produced in animal cell cultures. Transgenic plants are an interesting alternative for antibody production because of the reduced risk of mammalian viral ...

Purification of monoclonal immunoconjugates

This Application Note describes the use of several nonaffinity modes of chromatography for purification of immunoconjugates. Although individual monoclonal antibodies are very homogenous with respect ...

Rapid optimisation and development of an automated two-step purification procedure for monoclonal IgG antibodies; ÄKTAFPLC

This application note describes the development and optimisation of an automated two-step purification process for mouse monoclonal IgG antibodies (MAbs) on ÄKTAFPLC. Cell culture supernatants ...

Selective removal of aggregates with Capto adhere - Process-scale antibody purification

This application note describes the selective removal of antibody dimers and aggregates from a two-step process based on MabSelect SuRe™ and Capto™ adhere. Capto adhere is a strong anion exchanger ...

Using an autoclavable ultrafiltration cartridge for the production of monoclonal antibodies

A variety of in vitro bioreactor cartridges is available for the development of monoclonal antibodies (MAbs). Each is inlaid with hollow fibers to provide an exchange surface for nutrient media, gas, ...