GE Healthcare Life Sciences - Application notes

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Purification Centre
Applications

Histidine-tagged protein purification
MAb
Peptides
Application notes
Standard purification protocol for Peptides from Natural Sources
Standard purification protocol for Protein fragments
Standard purification protocol for Synthetic Peptides
Proteins
Rec Proteins

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Efficient isolation of protein fragments for structure analysis; ÄKTApurifier

A novel, high pressure, high performance chromatography system, ÄKTA ™ purifier and UNICORN ® , was used for efficient isolation of protein fragments for structure analysis. The pre-programmed ...

Highly sensitive phosphopeptide analysis using Ettan MDLC and a linear ion trap mass spectrometer

Ettan™ MDLC coupled to a linear ion trap mass spectrometer was used to characterize phosphopeptides in the low femtomole range. Phosphopeptides in a complex biological sample were sequenced, and the ...

Peptide Mapping at Low and High pH Using a Polymer Stationary Phase

Routinely, for protein structure characterisation, the peptide mapping step involves separation of the protein fragments by means of reversed phase chromatography on silica C8 or C18 sorbents using ...

Purification of a Phosphorylated PDGF alpha-Receptor Derived Peptide of High pH using Polymer Stationary Phase

Peptide pY574a is an 18 amino acid residue phosphorylated peptide constituting part of the intracellular domain of the PDGF a-receptor, which is involved in signal transduction. The peptide was ...

Purification of Amyloid-ß 1-42 at High pH using a Polymer Stationary Phase

Amyloid-b 1–42 is a peptide which is generated from a large transmembrane precursor protein by proteolytic cleavage. The peptide may polymerise to form rigid, linear, non-branching fibrils building ...

Purification of peptides from natural sources: Isolation and characterization of cholecystokin-58 (CCK-58) from pig intestine: ÄKTApurifier

A method is described for purifying a 58-residue molecular form of cholecystokinin, CCK-58, from porcine intestinal tissue using ÄKTA™ purifier system controlled by UNICORN ® . After extraction of ...

Purification of Synthetic peptides by RPC; ÄKTAexplorer

A general strategy for purification of synthetic peptides was used for rapid method optimisation and scale up, using ÄKTAexplorer and UNICORN ™. The strategy is described in detail for the ...