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Q. What is the concentration of cysteine and methionine in Pro-Mix formulations? A. The Methionine / Cysteine ratio in Pro-Mix products is approximately 70:30. Therefore on the reference date, the ...
Q. What is different about the SP6/T7 grade of radiolabeled ribonucleotides? A. Our SP6/T7 grade radionucleotides have been specifically formulated for use in RNA labeling experiments using SP6 or T7 ...
Q. Which of the stabilized methionine formulations is least cytotoxic to sensitive cells? A. The Redivue methionine formulation (AG1094, AG1594) has been found to be less cytotoxic to sensitive cells ...
Q. What is the chemistry of labelling by 35S-Sulfur Labelling Reagent (35SLR)? A. 35SLR , or "Sulfur-35 Labelling Reagent" is the simplified brand name we use for t-Butoxycarbonyl-L-[35S]-methionine, ...
Q. Should I use 3H-Amino Acids in aqueous solution or 2% ethanol? A. Most 3H-labeled amino acids have better stability in ethanolic solutions since ethanol acts as a reactive oxygen radical ...
Q. Why do 3H-labeled compounds decompose so quickly, and how can I estimate the shelf-life expected for a 3H-labeled radiochemical? Shelf Life, Usable Period, and Purity
We aim to put 3H (and 14C) ...
Q. What does the letter "U", "n", or "G" indicate when it precedes the position of the radiolabel in a radiochemical product name? A. These are abbreviations indicating the type and/or position of ...
Q: What is the difference between the translation grade and cell labeling grade [35S]-Methionine products? A: SJ1015 and SJ1515 are made from the same batch production of 35S-methionine and differ ...
Q. Can Redivue and other nucleotides stabilized with reducing agents be used with Terminal Deoxynucleotidyl Transferase (TdT) for 3'-end labelling DNA? A. High content SH-groups (DTT, ß-ME, or ...
Q: Is the Redivue dye and stabilizer chemically attached to radiolabeled product? A: The identity of stabilizer and dye used in the Redivue formulated radioactive products is proprietary. However, ...
Q: Can the Redivue formulation of radioactive products be stored frozen? A. Although Redivue formulated products can be stored at +4°C, they can also be stored frozen. If stored frozen, the ...
Q. Why are Redivue nucleotides shipped at ambient temperature? A. Redivue nucleotides are quite stable at room temperature, but it is generally recommended that they be stored at 4oC upon receipt. We ...
Q. Will the 126-I content of IMS30/IMS300 (Sodium 125-I Iodide), have any effect on labeling efficiency? A. 126-I will have no effect on the labeling efficiency of 125-I, but it should be noted that ...
Q. What is the role of pyridine 3,4-dicarboxylic acid in some of the 35S amino acid formulations? A. Pyridine 3,4-dicarboxylic acid (PDCA) is a stabilizer that is added to help minimize and slow ...
Q. What factors affect labeling efficiency using Pro-mix [L-35S] in vitro cell labeling mix? A. Labeling efficiency can be influenced by a number of factors including: 1) Cell Plating Density and ...
Q. What are the main impurities in Pro-Mix? A. Pro-Mix Cysteine/Methionine formulation has no cellular debris, unlike other commercialformulations. In addition, Pro-Mix has very low amounts of ...