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Amplify —References

Amplify (NAMP100) - References Godding, J., Monoclonal antibodies principles and practice, p.179-181, 2nd edition Perng, G., Rulli, R.D., Wilson, D.L., and Perry, G.W. " A comparison of fluorographic ...

Amplify – Gain in Sensitivity

Q. What is the gain in detection sensitivity when using Amplify fluorographic reagent? 3H: up to 1000x for acrylamide gel / 100x for paper, blot, agarose, or TLC 35S/14C: up to 15x for acrylamide ...

Autoradiography Film — Exposure Temperature and Sensitivity

Q. At what temperature should autoradiography film be exposed? A. In situations where there are very low amounts of radioactivity, or when fluorography reagents or intensifying screens are being ...

Microscale Autoradiography Standards — Polymer Density

Q. What is the density of the polymer that makes up the Microscales Autoradiographic Standards? A. The polymer density for the microscales ranges from 1.1-1.2. Click here for more information about ...

Microscale Autoradiography Standards — Solvent Stability

Q. Which solvents are known to affect the polymer matrix of microscales? A. Solvents such as acetone, chloroform, toluene, ethyl acetate, ether, acetonitrile, and xylene should be avoided as they can ...

Hypercoat Nuclear Emulsions — Number of Slides Coatable

Q: How many slides will Hypercoat EM-1 and LM-1 Nuclear Emulsions coat? A: Generally, each 40 mL volume of the Hypercoat Nuclear Emulsions should coat at least 50 slides. A number of factors may ...

Hypercoat Nuclear Emuslions — Blank/Uncoated Slide Areas

Q: When pre-coating microscope slides with Hypercoat Nuclear Emulsions, why do I occassionally see blank or uncoated areas? A: The blank areas or spots are called repellency spots. These are caused ...

Hyperfilm ECL — Use in Radioactive Applications

Q. Can Hyperfilm ECL be used for detection of radioisotopes? A. Hyperfilm ECL can be used for the detection of 3H, 14C, 35S, 33P, 32P, and 125I. Hyperfilm MP is quality controlled for use with ...

Hyperfilm MP and ECL — Film Processing Reagents

Q. What developer and fixer reagents are suitable for processing Hyperfilm MP and Hyperfilm ECL autoradiography films? A. Ilford D19, Dupont 30D and GAF30, May and Baker Escprol and Perfix reagents, ...

Hyperfilm MP — Use in Chemiluminescent Applications

Q. Can Hyperfilm MP be used for detection of chemiluminescence? A. Each batch of Hyperfilm MP is quality control tested in a radioactive application, while Hyperfilm ECL is quality control tested ...

Microscale Autoradiography Standards — Chemical composition of the microscale polymer

Q. What is the chemical composition of the microscale polymer? A. The polymer used to make all microscales (14C, 3H, and 125I) consists of butyl methacrylate: methyl methacrylate: ethylene glycol and ...

Microscale Autoradiography Standards — Quantitating 33P-labeled sections using 14C microscales

Q. Is it possible to quantitate 33P-labeled sections using 14C autoradiographic microscales? A. Yes, it is possible to use 14C microscales for 33P quantitation, but we do not have internal validation ...

Microscale Autoradiography Standards — Re-use

Q. Can the Microscale Autoradiography Standard strips be re-used? A. Yes, the microscale strips can be re-used many times. To remove the microscales from a slide, put the slide in 50�C water bath to ...

Microscale Autoradiography Standards— Calculation of Actual Tissue Activities

Q. How do I calculate actual tissue activity when using Microscale Autoradiography Standards? A. Microscales are meant to be exposed to film, nuclear emulsion slide coatings, or phosphorimaging. We ...

Microscale Autoradiography Standards — Storage Temperature

Q. At what temperature should Microscales be stored? A. Microscales should be only be stored at room temperature or refrigerated since storage at freezing temperatures will cause the polymer to ...

Microscale Autoradiography Standards—How to Attach Microscales to Slides

Q. How do I attach or adhere Microscale Autoradiography Standard strips to my slides? A. To attach a microscale to a slide, follow these directions: 1. Add one drop water to the slide and place the ...

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