Recommended Equipment
The LEADseeker™ Multimodality Imaging System CCD imager can measure SPA Imaging assays, Cy Dye fluors, HitHunter™ chemiluminescent and fluorescent end point Enzyme Fragment Complementation Assays as well as a wide range of fluorescent and luminescent tags in 96, 384 and 1536 well microplates. Appropriate filter sets are available for all these end points.
SPA Scintillation Beads can be counted on a range of scintillation counters. For counters fitted with spectrum analysis packages, the suitable window opening can be determined and the instrument set accordingly.
For further information regarding programming and calibration of counters, please contact your instrument manufacturer.
Counter type | Isotope and SPA bead type |
3H | 125I | 33P | 35S | 14C |
PVT | YSi | PVT | YSi | PVT | YSi | PVT | YSi | PVT | YSi |
Packard TopCount | 1.5-35 | 0-50 | 1.5-100 | 0-100 | 2.9-256 | 2.9-256 | 2.9-256 | 2.9-256 | 2.9-256 | 2.9-256 |
Wallac MicroBeta | 5-360 | 5-500 | 5-530 | 5-650 | 5-650 | 5-750 | 5-600 | 5-700 | 5-650 | 5-750 |
Hidex Chameleon* | 20-120 | 20-120 | 20-900 | 20-900 | 20-600 | 20-600 | N.D | N.D | N.D | N.D |
Beckman LS7800 | H# is set at 0 and the window is wide open from 10 to 999 |
Packard Tricarb 460 | Window open from 0 to 999 |
LKB 1209/1215 RackBeta | Window open from 5 to 999 |
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Instruments settings for SPA Scintillation beads - MicroBetaTM |
 | PVT | YSi |
isotope | window setting | efficiency (%) | window setting | efficiency (%) |
3H | 5 - 320 | 55 | 5 - 500 | 80 |
33P | 5 - 600 | 60 | 5 - 750 | 37 |
35S | 5 - 600 | 57 | 5 - 700 | 52 |
125I | 5 - 530 | 49 | 5 - 650 | 87 |
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Instruments settings for SPA Scintillation beads - TopCountTM |
| PVT | YSi |
isotope | window setting | efficiency (%) | window setting | efficiency (%) |
3H | 1.5 - 35 | 34 | 0 - 50 | 69 |
33P | 1.6 - 150 | 47 | 2.9 - 256 | 30 |
35S | 1.5 - 150 | 40 | 2.9 - 256 | 43 |
125I | 1.5 - 100 | 33 | 0 - 100 | 75 |
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Instrumentation Settings |
TopCount | Version 4 software and above | scintillator – liquid/plastic
energy range – low
mode – normal | scintillator - glass energy range - low mode - high sensitivity |
TopCount | Version 3 software and below | scintillator – liquid
energy range – low
mode – high | scintillator - solid energy range - low mode - normal |
Other instruments e.g. RackBeta or Tri-Carb Liquid Scintillation Analyzer | Applicable software | wide open | wide open |
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New to Scintillation Proximity Assay (SPA)? Get started with the basics
Use theSPA selection guide to see the entire product range
Understand the safety issues involved in using this technology
Visit the literature section and browse from over 800 references
Consult the technical support and customer service section for more support
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