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Scintillation proximity assay (SPA), is an innovative approach for assay development and biochemical screening that allows the rapid and sensitive measurement of a wide variety of molecular interactions in a homogeneous system.
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Introduction to SPA
SPA Beads are microscopic beads which contain a scintillant that can be stimulated to emit light. This stimulation event only occurs when radiolabeled molecules of interest are bound to the surface of the bead then blue light is emitted that can be detected on standard scintillation counters
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Energy absorbed by medium no light generated | Bound radiolabel, bead stimulated to emit light |
A wide range of bead coatings allows specific bead types to be manufactured for specific applications including; receptor-ligand binding, enzyme assays, radioimmunoassays, protein-protein and protein-DNA interactions.
Are you currently using coated plate based methods?
SPA bead-based assays have many advantages over coated plate-based methods:
- The higher surface area of SPA beads allows the concentration of key assay components (e.g. receptor membrane) to be increased or decreased, thus giving greater flexibility in assay design.
- The higher binding capacity of SPA beads gives higher signal allowing a potential reduction in the amount of radioactive label required. The amount of SPA bead can be altered to optimize the sensitivity of an assay.
- SPA beads are format independent making it easier to transfer assays to higher density plates.
- A choice of four different core bead types allows optimization of signal and background over a wide range of applications
- SPA beads are not dependent on one plate type or supplier
Use the SPA product selection guide to see the entire product range
Understand the safety issues involved in using this technology
Do you have the right equipment for this technology?
Visit the literature section and browse from over 800 references
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