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Desalting & gel filtration - Technique in which low-molecular-weight salts and other compounds are removed from non-ionic and high-molecular-weight compounds.
Gel filtration separates mixtures of biomolecules according to size. Large molecules elute either in the void volume or early in a chromatographic separation. Smaller molecules elute later, depending on their degree of penetration of the pores of the matrix. Gel filtration is a simple and mild technique which complements ion exchange, hydrophobic interaction and affinity chromatography.
In process-scale chromatography, gel filtration is principally used for desalting the product, for buffer exchange, for rapid removal of reagents to terminate a reaction, or for specific removal of contaminants with molecular weights above or below that of the desired product. Typically, molecules must differ in size by 2-fold to yield a good separation, although other adsorptive effects can augment some separations where molecules are similar in size.
Gel filtration is useful for at the polishing stage where volumes are much lower than at the capture or intermediate stages and there is a need to remove dimers or aggregates. Gel filtration separates mixtures of biomolecules according to size. Large molecules elute either in the void volume or early in a chromatographic separation. Smaller molecules elute later, depending on their degree of penetration of the pores of the matrix. Gel filtration is a simple and mild technique which complements ion exchange, hydrophobic interaction and affinity chromatography.
Read more about gel filtration chromatography.
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