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UPDATED - Recombinant Protein Purification – Principles and Methods
GE Healthcare’s Recombinant Protein Purification Handbook is a comprehensive, practical guide reflecting new innovations in the rapidly developing field of recombinant protein expression and purification. The revised handbook covers the following topics in detail:
- Effective sample preparation to maximize recombinant protein expression
- Manual purification of tagged proteins, as well as automated purification using ÄKTAdesign™ chromatography systems
- Optimization of histidine- and GST-tagged protein purification
- Purification using new prepacked columns, gravity-flow, spin columns, and multiwell plates from GE Healthcare
- Inclusion bodies and how to overcome them
- In addition to theoretical considerations, application examples illustrate recovery and purification of recombinant proteins from different sources and hosts.
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The handbook also provides hints, tips, and a troubleshooting guide to help you make your recombinant protein purification more effective.
The use of recombinant proteins has increased greatly in recent years, in step with a number of new techniques and products used for their expression and purification.
Download Recombinant Protein Purification Handbook: Principles and Methods (login required)
or click here to view our complete selection of handbooks and to order printed copies.
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