Recombinant proteins

The design of efficient, economic and safe purification processes for proteins and peptides produced using recombinant DNA technology is a major challenge in bringing new biopharmaceuticals to the market.

Purification problems vary depending upon the source and location of the product. Extracellular sources from microorganisms or mammalian cell lines present relatively few impurity problems and products are usually stable unless there is extensive cell lysis. Information about expression systems is summarized below.

Regardless of the production source (expression system type), a process normally follows the Capture-Intermediate Purification-Polishing, a recommended approach to address the overall goals of the downstream purification. This often results in a process containing three to five chromatographic purification steps. It is important to link different chromatographic techniques and steps optimally in order to achieve a robust and scalable process design prepared for industrial demands.

Read more about how to produce recombinant proteins with bacteria, insect cells, mamalian cells, transgenics, and yeast.