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ADME Rat 16-Assay Bioarrays for Gene Expression
CodeLink™ ADME Rat 16-Assay Bioarrays are designed to deliver biologically relevant results for ADME and toxicology studies. Each bioarray contains 16 arrays—each with ~1280 single-stranded oligonucleotide probes derived from publicly available, well-annotated mRNA sequences found in the Rat UniGene set of unique clusters. Each sequence was carefully screened to reduce gene redundancy and design high-quality, specific probes. Every probe is functionally validated to ensure reliable performance for ADME and toxicological applications.
CodeLink ADME Rat 16-Assay Bioarray utilizes the multiassay bioarray format and is currently available as a catalog item.
This information is also available in Poster format (PDF) | .. |  |
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Specifications
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| Number of bioarrays per package | 1 or 3 |
| Number of arrays per 1 x 3-in bioarray | 16 |
| Number of probes per array | 1280 |
| Number of transcripts | 1290 |
| Number of discovery genes | 1223 |
| Positive controls | 18 |
| Negative controls | 18 |
| Number of probes per gene | one specific and functionally-validated probe |
| Oligo probe length | 30-mer |
| RNA sample input | 0.2–2 µg total RNA |
| Hybridization assay parameters | 60 µl hybridization volume and 1 µg labeled-cRNA target per assay per well |
| Type of assay | one color |
| Storage and handling temperature | room temperature |
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CodeLink multiassay bioarray
CodeLink multiassay bioarray features a new multiassay chamber that allows up to 16 samples to be hybridized, washed, and detected in parallel on a single slide (Fig 1). Especially for high-volume microarray users who require flexibility for custom arrays, this latest CodeLink bioarray format offers increased throughput and cost efficiencies with a robust experimental protocol.
16 identical arrays spotted on each bioarray slide and separated by individual hybridization chambers.
High throughput in a robust assay that can be automated. With three bioarrays used together on a microtiter tray, 48 arrays can be processed as a single unit, making this format ideal for automation.
Open system format
The multiassay bioarrays can be detected by scanning using commonly available microarray scanners. Images generated can be easily analyzed with CodeLink Expression Analysis software and the data further visualized and mined with commercially available microarray data analysis applications.
CodeLink multiassay bioarray delivers superior results through proven CodeLink core technologies and processes:
- Quality probe design and selection that includes functional validation for accurate and reproducible biological results
- Unique 3-D aqueous gel matrix for higher assay sensitivity and specificity
- Six Sigma manufacturing processes and control checkpoints to ensure probe and deposition quality for reproducible experimental results
- Optimized reagents, protocols, processing tools, and software for ease of use and reliable performance
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 | Typical performance | Benefit |
| Sensitivity | Mass ratio <1:1 000 000 with bacterial spiking into cRNA or total RNA level | Detection of more
low abundance transcripts (<0.3 copies per cell) |
| Dynamic range | Linear signal response across three orders of magnitude of concentration (0.05 pM – 50 pM) | More usable/accurate data across a broad range of gene expression levels |
| Specificity | Discrimination of >2 base mismatches | Better discrimination of highly homologous genes |
| Signal reproducibility | Typically <15% total median CV among production batches, all probes included; ~8% median CV among production batches, only probes above noise included | Reproducible differential
expression results in every experiment |
| Minimum detectable fold change | >98% within two-fold change for replicates | Reduced noise for better
detection of small changes in gene expression and more usable data per bioarray |
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Fig . CodeLink multiassay bioarrays exhibit high signal reproducibility.
This experiment used all 16 arrays across two slides. The same target—mouse heart with bacterial spikes at 1:300 000 level—was used on all arrays. The blue line is a fitting line using lognormal-type fitting method. |
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High data correlation between CodeLink bioarray formats
CodeLink multiassay bioarrays exhibit excellent signal and ratio correlation with the CodeLink single-assay bioarrays that utilize the Flex Chamber. This allows for data comparison between catalog bioarrays and custom multiassay bioarrays.
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