CyScribe Microarray Labeling Kits are designed for generating probes for dual-color differential gene expression analysis on microarrays. Although unprocessed data can demonstrate the presence of differentially expressed transcripts, careful normalization of quantified data is required to achieve meaningful results.

Equally expressed genes

Signal from differentially expressed genes Genes expressed at low levels Cy3 signal is represented by green spots Cy5 signal is represented by red spots

Fig 1. Close-up of a microarray slide hybridized with Cy3-labeled muscle cDNA and Cy5-labeled brain cDNA. Equal amounts of both cDNAs (pmol of dye) were hybridized together on a microarray slide containing immobilized gene targets.