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Lucidea Spotting Pens
| Spot-to-spot reproducibility is essential for quantitative comparison of genes on an array. To achieve good spot uniformity within a slide and across multiple experiments, the pens within each set are matched to tight specifications.
Each pen is individually spring-loaded to create better deposition and spot uniformity while extending pen lifetime and maintaining high speed and throughput. | .. | |
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| Specifications |
| Deposition configuration | 24 spring-loaded, stainless steel capillary pen design |
| Pen load volume | < 200 nl uptake volume; .1 nL delivery volume |
| Nominal spot diameter | ~140-160 µm |
| Pen cleaning | Integrated, active wash system flushes pens using optimized solutions and vacuum dry |
| Wash solutions | Wash 1: 0.01% SDS
Wash 2: 0.05M NaHCO3
Wash 3: DI Water (17 megohm/cm or better) |
| Wash efficiency | No detectable dye carryover, as determined using radioactive detection methods |
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Lucidea Spotting Pen Set Instructions
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