Reagents for hybridization
Hybridization buffer contains a number of components to assist in hybridization:
- Rate enhancers to exclude volume and increase the rate and extent of hybridization.
- Detergents and other blocking agents to reduce non specific binding of the probe to the membrane
- Denaturants decreasing the melting temperature of the probe so that reduced temperatures can be used.
- Heterologous DNA to reduce non specific binding of probes to non-homologous DNA on the blot.
Consult the technical support section for tips or contact information
Select a product from another hybridization product range
See our product selection for each Southern and Northern blotting workflow step
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