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Southern & Northern Blot Home
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Electrophoresis
Blotting
Labeling
Hybridization
Reagents for Hybridization
Rapid-Hyb Buffer
Sonicated Salmon Sperm DNA
Sonicated Calf Thymus DNA
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Detection
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Reagents for hybridization


Rapid-Hyb buffer

Enhances the hybridization rate of radioactive probes
Sonicated salmon sperm DNA

Reduce background caused by non-specific binding
Sonicated calf thymus DNA

Reduces background caused by non-specific binding


Hybridization buffer contains a number of components to assist in hybridization:
  • Rate enhancers to exclude volume and increase the rate and extent of hybridization.
  • Detergents and other blocking agents to reduce non specific binding of the probe to the membrane
  • Denaturants decreasing the melting temperature of the probe so that reduced temperatures can be used.
  • Heterologous DNA to reduce non specific binding of probes to non-homologous DNA on the blot.



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