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Characteristics of new short gel filtration columns

Gel filtration (GF) is a gentle technique suitable for the study of aggregation and purity of a target protein. GF, however, is often time consuming and sample and buffers can be expensive. A new, ...

Efficient Cleanup of Low Molecular Weight Carbohydrate Samples using Gravity Flow Columns

Low molecular weight carbohydrates (oligosaccharides) can be difficult to separate from contaminants such as salts. Methods like ion exchange are not suitable due to interference by the contaminant. ...

Efficient, rapid protein purification and on-column cleavage using GSTrap FF columns

A purification strategy involving the production of soluble, purified proteins in a single step would be an invaluableresource and indispensable in many laboratory environments. This Application Note ...

Fast and simple purification of histidine-tagged proteins using His GraviTrap

His GraviTrap gives the following performance benefits: Five-times faster purification than the comparable product from another supplier Possibility to purify both clarified and unclarified ...

Fluorescence Quantitation of Single-Stranded M113 DNA

Determination of the concentration of recombinant M13 DNA is important for automated and manual DNA sequencing, gel electrophoresis analysis, molecular cloning and site-directed mutagenesis. ...

New column format for process development

HiScreen™ columns are part of the process development platform available from GE Healthcare. The columns are prepacked with a range of BioProcess™ media and designed for method optimization and ...

Rapid protein analysis and screening of membrane protein buffer conditions by gel filtration

Screening of different conditions is often necessary to obtain protocols that result in pure and stable target proteins. Sizeexclusion chromatography (SEC) is an excellent and gentle technique to ...

Rapid screening of antibody aggregates and protein impurities using short gel filtration columns

Purification of antibodies often generates dimers and higher aggregated forms and during optimization of purification protocols, many samples may need to be analyzed for aggregate content. Gel ...

Snags with tags: Some observations made with (His)6 -tagged proteins

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The Trap platform and Tecan automation – efficient solutions for screening proteins

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