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A Method for Representatively Amplifying Genomic DNA, Genomic/Proteomic Technology April/May 2003

Reprinted from Genomic/Proteomic Technology April/May 2003 Representational amplification of human genomic DNA from a small number of cells or a limited amount of a precious sample can be extremely ...

A new process for supercoiled plasmid DNA purification (Downstream 35, 2002)

Plasmid DNA intended for clinical applications must meet stringent purity requirements. If it is then to be used commercially, the purification method employed must also be suitable for largescale ...

Amplification, cloning, and expression of an exonuclease III gene from a thermophilic bacterium without culturing

Genomic DNA from a glycerol stock of Thermoplasma volcanium was amplified using GenomiPhi™ DNA Amplification KitG. The amplified DNA was used to create a whole genome shotgun library that was ...

Automation of TempliPhi and GenomiPhi DNA Amplification Kits on the Genesis Freedom Robotic Sample Processor (from Discovery Matters Issue 2, October 2005)

GE Healthcare offers a range of DNA amplification products to meet all genomic DNA and sequencing template preparation needs. A combination of these kits with the Genesis Freedom™ Robotic Sample ...

Generation of microgram quantities of DNA from plant samples with GenomiPhi DNA Amplification Kit (from Discovery Matters, July 2005)

GenomiPhi™ DNA Amplification Kit offers a way to prepare microgram quantities of DNA from leaves and seeds. Often a simple lysis is sufficient to prepare the DNA for amplification. In plants ...

Genomic DNA preparation using illustra GenomiPhi V2 and HY DNA Amplification Kits (Discovery Matters 6, Aug 2007)

The illustra™ GenomiPhi™ V2 and HY DNA Amplification Kits are the latest additions to the Phi 29 DNA polymerase family of products from GE Healthcare. GenomiPhi V2 DNA Amplification Kit yields 4–7 μg ...

Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases (from Discovery Matters Issue 2, October 2005)

Competitive binding assays have been developed for measuring the functional activity of a range of serine-threonine and tyrosine kinases. Based on enzyme fragment complementation and the subsequent ...

Increased PCR amplification specificity using illustra Hot Start Master Mix (from Discovery Matters Issue 4, June 2006)

We highlight the hot start capability of illustra™ Hot Start Master Mix, providing evidence of its polymerase-blocking ability and elimination of primer-dimer formation.

Low-cost, automated serial purification of histidine-tagged proteins (Discovery Matters 6, Aug 2007)

Automated chromatographic systems are of great help to structural genomics programs involved in expressing and purifying numerous recombinant proteins. A low-cost and automated process, using ...

Isolation of bacterial genomic DNA using Nucleon BACC 1 (from Life Science News, issue 1)

RFLP analyses are being used to study the epidemiology of Legionella pneumophila serogroup 1 associated with outbreaks of Legionnaire’s disease. The AFLP patterns help to establish the relationships ...

Isolation of DNA from cryostat sections of bone using Nucleon BACC 1 (from Life Science News, issue 1)

Osteocytes undergo apoptotic cell death under certain circumstances which may contribute to the control of bone modelling and re-modelling. One characteristic used in the study of apoptosis is the ...

Isolation of DNA from sperm using Nucleon BACC 2 (from Life Science News, issue 1)

Using an adaptation of the Nucleon™ BACC 2 protocol has enabled the optimization of DNA yields from sperm samples from infertile patients with probable low sperm counts.

High-throughput genotyping (from life science news 4)

Genotyping is used to help determine which genes are responsible for specific inherited traits. Identifying these genetic markers can help researchers understand the mechanisms behind certain, ...

Rapid screening of PCR products using a novel agarose gel electrophoresis system (from Life Science News, issue 6)

Ready-To-Run Electrophoresis System is designed for the rapid screening of large numbers of PCR products. The modular system features precast gel cassettes; a Separation Unit with built-in power ...

New oligonucleotide probes for validating and normalizing microarray gene expression data (from Discovery Matters, July 2005)

Oligonucleotide probes corresponding to each of the exogeneous genes for Universal ScoreCard™ have been developed to create a new set of controls for microarrays. Functional testing using microarray ...

Redesigned HiTrap fittings offer easier connection to AKTAdesign chromatography systems (Life Science News 18, p19)

The new inlet and outlet design of HiTrap columns eliminates the need for column connectors when used with �KTAdesign™ chromatography systems. Connection to a syringe, laboratory pump, or ...

Scintillation proximity assays for drug metabolism and pharmacokinetic applications (from Life Science News 13, 2003)

High-throughput screening (HTS) for new pharmaceuticals has been strongly driven by the need to test large numbers of novel compounds against increasing numbers of new drug targets. Consequently, ...

Snags with tags: Some observations made with (His)6-tagged proteins

The unusual behavior during purification of four affinity-tagged proteins—a MAP kinase, a transcription factor, intracellular binding protein, and a receptor tyrosine kinase—led the authors to ...

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