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The GST 96-Well Detection Module uses immobilized polyclonal anti-GST antibody for capture of GST fusion proteins. Captured proteins are detected using a horseradish peroxidase/anti-GST conjugated ...
The hydrophobic nature of membrane proteins generally makes them more difficult to study than soluble proteins. Detergents are used for solubilization of membrane proteins and must be present in all ...
Protein G HP MultiTrap™ was used to enrich human alpha-transferrin (hTf) more than 100-fold from E. coli extract. The eluate resulting from enrichment was analyzed by SDS-PAGE or LC-MS, and the yield ...
The unusual behavior during purification of four affinity-tagged proteins—a MAP
kinase, a transcription factor, intracellular binding protein, and a receptor
tyrosine kinase—led the authors to ...
Immobilized Metal Affinity Chromatography (IMAC) is an excellent chromatography technique for purification of histidine-tagged proteins. Pure protein can be obtained from a cell extract in a single ...
An efficient and rapid protein purification and on-column cleavage strategy using GSTrap Columns