Plasmid Amplification using Rolling Circle Amplification

illustra TempliPhi 100/500 DNA Amplification Kit Generate microgram quantities of template DNA from picogram amounts of starting material. Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks, or purified circular (plasmid or M13) DNA. High quality DNA in only 4 hours - Eliminating the need for overnight culture. illustra TempliPhiDNA Sequencing Template Preparation Kit Generate microgram quantities of template DNA from picogram amounts of starting material. Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks, or purified circular (plasmid or M13) DNA. High quality DNA in only 4 hours - Eliminating the need for overnight culture. illustra TempliPhi Large Construct Kit Developed for high-throughput users and specifically designed for BAC or fosmid DNA preparation. Generate 5 µg amounts of total DNA. Simple, automation-friendly protocol with great reproducibility. illustra TempliPhi Sequence Resolver Kit Amplify difficult templates for successful DNA sequencing, while reducing repeats and sequencing stops with a single kit. Save time, only 20 min hands-on time, and reagents with cultureless preparation from bacterial colonies, glycerol stock or puriried DNA. Improve difficult template sequencing success with up to 820 bp Phred 20 read length.

See an animation explaining rolling circle amplification.

Find TempliPhi citations on Google Scholar: Click here to search. Read the Methods: Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification Genome Res. - Vol. 11, Issue 6, 1095-1099, June 2001