Frequently Asked Questions
What are the advantages of GenomiPhi™ over conventional DNA sample purification or amplification methods, and workflow?
The biggest advantage is an almost unlimited supply of DNA for genetic studies. Amplification of genomic DNA from simple lysates generates representative, high fidelity products that can be used directly in various genetic studies. With less than twenty minutes of hands-on time and a simple protocol there are significant savings on DNA purification kits, consumables, waste disposal costs, and overall genomic DNA preparation time. .
What starting material can I use with GenomiPhi kits?
Any sample of good quality DNA is a suitable substrate for amplification including frozen tissue, blood, buccal and cell cultures. Degraded DNA such as from formalin-fixed, paraffin-embedded tissues is not a good starting material for amplification.
Is the amplified DNA representative of the input DNA?
Yes as long as sufficient amounts (at least 10 ng) of good quality DNA are used per amplification reaction. Various genotyping studies have shown that the amplified DNA is over 99% concordant with genomic DNA.
Why do I get an amplification product when I don’t add any DNA?
In the absence of input DNA an amplification product will still be observed. The product is non-specific, resulting from amplification of the hexamers in the reaction. It will not respond to gene specific PCR or perform in downstream applications. |
