GenomiPhi protocol
Download the GenomiPhi instructions:
The GenomiPhi™ DNA Amplification Kit uses a simple, automatable and labor saving protocol.The starting genomic DNA is combined with a sample buffer containing random hexamer primers. The mixture is heat denatured and cooled to allow random priming of the hexamers.
Then, the remaining reaction components— including Phi29 DNA polymerase, deoxynucleotide triphosphates, and buffer components optimized for linear DNA synthesis—are added. This reaction mixture is incubated overnight at 30° C, during which time the available nucleotides are consumed and converted into high molecular weight fragment copies of the input DNA.
Sample input amount
> 10 ng human genomic DNA or equivalent
| Yield
4-7 µg from purified genomic DNA
|
Total hands on time
< 20 min | Reaction time
Overnight |
 |
